Detect cell migration and invasion in a homogeneous fluorescent assay system
Corning FluoroBlok Cell Culture Inserts are designed with a unique light-tight PET membrane that efficiently blocks the transmission of light within the range of 400-700 nm.
Fluorescently labeled cells present in the top chamber of the insert are made invisible by the Corning FluoroBlok membrane.Once labeled cells migrate through the membrane, they are no longer shielded from the light source and are easily detected with a fluorescence plate reader.
◗ Simplify insert-based assays—Unique, light-tight PET membrane makes it easy to specifically detect fluorescently labeled cells and molecules below the insert.
◗ Increase insert assay productivity—Save time and labor in chemotaxis, cell migration, and invasion assays by automating your assay detection with real-time fluorescence.
◗ Eliminate cell culture insert manipulation—Get rapid data collection using a fluorescence microplate reader or microscope without the need for plate washing or tedious, manual cell scraping and counting. Chart migration of cells and molecules in real time without dismantling or destroying the insert.
Applications
Corning FluoroBlok effectively blocks >99.0% of the light transmission from 400-700 nm, allowing you the flexibility to choose from a variety of fluorophores for bioavailability, toxicity, chemotaxis, cell migration, and tumor invasion assays. As long as the fluorophores of choice are within the blocking range of the Corning FluoroBlok membrane, multiplex detection is also possible.
